neb 10x kld (New England Biolabs)
99
Structured Review
New England Biolabs
neb 10x kld
Neb 10x Kld, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neb+10x+kld/DpnI/pm41986324-190-31-31
Average 99 stars, based on 6159 article reviews
Neb 10x Kld, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neb+10x+kld/DpnI/pm41986324-190-31-31
Average 99 stars, based on 6159 article reviews
neb 10x kld - by Bioz Stars,
2026-10
99/100 stars
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Amplification:Article Title: Acclimation of Photosynthesis to the Environment 1 acts as a copper-binding superoxide detoxification enzyme. Article Snippet: Briefly, pLIC03-APE1 was amplified via PCR using NEB Q5 High-Fidelity 2X Master Mix in 25 μl reaction volumes containing 10 ng DNA template and 0.5 μM of specific primers pairs carrying the new codon (C128S mutation: TGC-AGC; H163A: CAC-GCG; C247S: TGC-AGC); the PCR protocol was set according to the manufacturer's instructions (amplification was done for 25 cycles with 180 s elongation time each) and annealing temperatures were calculated with the online NEB Tm calculator tool. .. After amplification, to remove the initial DNA template (i.e., without the added mutation) and ligate the PCR products, 1 μl of PCR product was incubated at RT for 10 min with Mutagenesis:Article Title: Acclimation of Photosynthesis to the Environment 1 acts as a copper-binding superoxide detoxification enzyme. Article Snippet: Briefly, pLIC03-APE1 was amplified via PCR using NEB Q5 High-Fidelity 2X Master Mix in 25 μl reaction volumes containing 10 ng DNA template and 0.5 μM of specific primers pairs carrying the new codon (C128S mutation: TGC-AGC; H163A: CAC-GCG; C247S: TGC-AGC); the PCR protocol was set according to the manufacturer's instructions (amplification was done for 25 cycles with 180 s elongation time each) and annealing temperatures were calculated with the online NEB Tm calculator tool. .. After amplification, to remove the initial DNA template (i.e., without the added mutation) and ligate the PCR products, 1 μl of PCR product was incubated at RT for 10 min with Polymerase Chain Reaction:Article Title: Acclimation of Photosynthesis to the Environment 1 acts as a copper-binding superoxide detoxification enzyme. Article Snippet: Briefly, pLIC03-APE1 was amplified via PCR using NEB Q5 High-Fidelity 2X Master Mix in 25 μl reaction volumes containing 10 ng DNA template and 0.5 μM of specific primers pairs carrying the new codon (C128S mutation: TGC-AGC; H163A: CAC-GCG; C247S: TGC-AGC); the PCR protocol was set according to the manufacturer's instructions (amplification was done for 25 cycles with 180 s elongation time each) and annealing temperatures were calculated with the online NEB Tm calculator tool. .. After amplification, to remove the initial DNA template (i.e., without the added mutation) and ligate the PCR products, 1 μl of PCR product was incubated at RT for 10 min with Incubation:Article Title: Acclimation of Photosynthesis to the Environment 1 acts as a copper-binding superoxide detoxification enzyme. Article Snippet: Briefly, pLIC03-APE1 was amplified via PCR using NEB Q5 High-Fidelity 2X Master Mix in 25 μl reaction volumes containing 10 ng DNA template and 0.5 μM of specific primers pairs carrying the new codon (C128S mutation: TGC-AGC; H163A: CAC-GCG; C247S: TGC-AGC); the PCR protocol was set according to the manufacturer's instructions (amplification was done for 25 cycles with 180 s elongation time each) and annealing temperatures were calculated with the online NEB Tm calculator tool. .. After amplification, to remove the initial DNA template (i.e., without the added mutation) and ligate the PCR products, 1 μl of PCR product was incubated at RT for 10 min with Plasmid Preparation:Article Title: Acclimation of Photosynthesis to the Environment 1 acts as a copper-binding superoxide detoxification enzyme. Article Snippet: Briefly, pLIC03-APE1 was amplified via PCR using NEB Q5 High-Fidelity 2X Master Mix in 25 μl reaction volumes containing 10 ng DNA template and 0.5 μM of specific primers pairs carrying the new codon (C128S mutation: TGC-AGC; H163A: CAC-GCG; C247S: TGC-AGC); the PCR protocol was set according to the manufacturer's instructions (amplification was done for 25 cycles with 180 s elongation time each) and annealing temperatures were calculated with the online NEB Tm calculator tool. .. After amplification, to remove the initial DNA template (i.e., without the added mutation) and ligate the PCR products, 1 μl of PCR product was incubated at RT for 10 min with Isolation:Article Title: Acclimation of Photosynthesis to the Environment 1 acts as a copper-binding superoxide detoxification enzyme. Article Snippet: Briefly, pLIC03-APE1 was amplified via PCR using NEB Q5 High-Fidelity 2X Master Mix in 25 μl reaction volumes containing 10 ng DNA template and 0.5 μM of specific primers pairs carrying the new codon (C128S mutation: TGC-AGC; H163A: CAC-GCG; C247S: TGC-AGC); the PCR protocol was set according to the manufacturer's instructions (amplification was done for 25 cycles with 180 s elongation time each) and annealing temperatures were calculated with the online NEB Tm calculator tool. .. After amplification, to remove the initial DNA template (i.e., without the added mutation) and ligate the PCR products, 1 μl of PCR product was incubated at RT for 10 min with DNA Sequencing:Article Title: Acclimation of Photosynthesis to the Environment 1 acts as a copper-binding superoxide detoxification enzyme. Article Snippet: Briefly, pLIC03-APE1 was amplified via PCR using NEB Q5 High-Fidelity 2X Master Mix in 25 μl reaction volumes containing 10 ng DNA template and 0.5 μM of specific primers pairs carrying the new codon (C128S mutation: TGC-AGC; H163A: CAC-GCG; C247S: TGC-AGC); the PCR protocol was set according to the manufacturer's instructions (amplification was done for 25 cycles with 180 s elongation time each) and annealing temperatures were calculated with the online NEB Tm calculator tool. .. After amplification, to remove the initial DNA template (i.e., without the added mutation) and ligate the PCR products, 1 μl of PCR product was incubated at RT for 10 min with |